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目的通过对食源性疾病病例监测为食源性疾病诊断提供病原学确证,进一步探讨食源性疾病的治疗、预防和控制措施。方法按照国标方法进行几种致病菌的分离鉴定,按照CLSI 2010进行药敏试验操作及结果判定。结果 200份样品中共检出48株致病菌,检出率为24.00%。其中致泻性大肠埃希菌37株,沙门菌6株,副溶血性弧菌3株,志贺菌2株。分离出的志贺菌和副溶血性弧菌对13种抗生素全部敏感,分离出的沙门菌仅对四环素存在耐药性;分离出的大肠埃希菌中,有19株对13种抗生素均敏感,18株对8种抗生素具有一定的耐药性,产生10种耐药谱。结论通化市人民医院的食源性疾病患者粪便标本中致泻性大肠埃希菌检出率最高,其次为沙门菌,且两者均存在耐药株,应引起相关部门重视。
Objective To provide etiological confirmations for the diagnosis of food-borne diseases by monitoring food-borne disease cases and to further explore the treatment, prevention and control measures of food-borne diseases. Methods According to the national standard method, several pathogenic bacteria were isolated and identified. According to the CLSI 2010, the drug sensitivity test and the result determination were carried out. Results A total of 48 pathogenic bacteria were detected in 200 samples with a detection rate of 24.00%. Including 37 diarrhea Escherichia coli, Salmonella 6, Vibrio parahaemolyticus 3, Shigella 2 strains. Isolated Shigella and Vibrio parahaemolyticus were all sensitive to 13 kinds of antibiotics, isolated Salmonella resistant only to tetracycline; isolated Escherichia coli, 19 were sensitive to 13 kinds of antibiotics , 18 strains of 8 kinds of antibiotics have certain resistance, resulting in 10 kinds of resistance spectrum. Conclusions The detection rate of diarrhea-causing Escherichia coli in fecal samples of patients with food-borne diseases in Tonghua Municipal People’s Hospital is the highest, followed by Salmonella, and both of them have drug-resistant strains, which should be paid more attention by relevant departments.