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枳(Poncirus trifoliata L.)高抗柑橘衰退病病毒(Citrus tristeza virus,CTV),但相关抗性机理仍不为人所知。以嫁接健康枝条的无毒枳作为对照,用Affymetrix柑橘基因芯片分析了感染CTV后枳叶片中基因的表达变化情况。结果共检测到表达量差异倍数≥2的基因295个,其中216个表达上调,79个表达下调。BLAST2GO分析发现,差异表达的基因中与抗逆反应相关的基因最多,乙烯、茉莉酸、赤霉素、脱落酸、生长素、水杨酸等植物激素代谢和调节相关基因为数不少,一些编码细胞壁形成或组分相关蛋白的基因差异表达明显。本研究可为揭示枳抗CTV的机理提供了转录组学线索。
Poncirus trifoliata L. is highly resistant to Citrus tristeza virus (CTV), but the mechanism of related resistance is still unknown. Using non-toxic Trifoliate grafted with healthy branches as a control, the gene expression changes in the leaves of P. anisopliae infected with CTV were analyzed by Affymetrix citrus gene chip. Results A total of 295 genes were detected with a multiple of ≥2 in expression level, of which 216 were up-regulated and 79 were down-regulated. BLAST2GO analysis showed that most of the differentially expressed genes were related to anti-retrogradation genes. A large number of genes related to the metabolism and regulation of plant hormones such as ethylene, jasmonic acid, gibberellin, abscisic acid, auxin, and salicylic acid, Differentially expressed genes that form or are component-related proteins are significantly expressed. This study may provide a transcriptomic clues for revealing the mechanism of CTV against CTV.