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目的：探讨蜕皮甾酮（Ｅｃｄｙｓｔｅｒｏｎｅ，ＥＤＳ）对内皮细胞释放一氧化氮的影响。方法：体外培养人脐静脉内皮细胞，测定培养液亚硝酸盐含量。结果：５０～４００ｍｇ／Ｌ蜕皮甾酮增加培养上清液中ＮＯ２－含量，２００ｍｇ／Ｌ作用达到最高峰；但剂量在８００ｍｇ／Ｌ以上时则减少ＮＯ２－含量。ＥＤＳ对ＮＯ２－生成的影响的时效关系为：在２～４ｈ上清液ＮＯ２－的含量逐渐增加，在４小时后ＮＯ２－含量维持不变。结果：ＥＤＳ低浓度时促进内皮细胞释放ＮＯ，高浓度时抑制内皮细胞释放ＮＯ。
Objective: To investigate the effect of Ecdysterone (EDS) on the release of nitric oxide from endothelial cells. METHODS: Human umbilical vein endothelial cells were cultured in vitro and the nitrite content in culture medium was determined. Results: 50-400 mg/L quercetin increased NO2-concentration in the culture supernatant, and the effect of 200 mg/L reached the highest peak; however, when the dose was 800 mg/L or more, the NO2-content was decreased. The time-effect relationship of EDS on the NO2-production was: the NO2-content in the supernatant was gradually increased at 2 to 4 h, and the NO2-content was unchanged at 4 h. RESULTS: EDS promoted the release of NO from endothelial cells at low concentrations, and inhibited the release of NO from endothelial cells at high concentrations.