论文部分内容阅读
目的探讨T-bet,GATA-3和RORC在过敏性紫癜患者发病中的作用机制。方法采用SYBR GreenI实时荧光定量PCR法检测32例过敏性紫癜患者和15例正常人外周血单核细胞中T-bet,GATA-3和RORC mRNA的表达水平。结果过敏性紫癜患者与正常人T-bet基因ΔCt平均秩次值分别为27.70和16.10,患者组T-betmRNA表达水平低于对照组;过敏性紫癜患者与正常人GATA-3基因ΔCt平均秩次值分别为18.25和36.27,患者组GATA-3 mRNA表达水平高于对照组,过敏性紫癜患者与正常人RORC基因ΔCt值分别为2.00±0.46与3.29±1.57,患者组RORC mRNA表达水平高于对照组,差异均有统计学意义(P均<0.05)。结论转录因子T-bet,GATA-3和RORC可能在过敏性紫癜发病机制中具有重要作用。
Objective To investigate the mechanism of T-bet, GATA-3 and RORC in the pathogenesis of allergic purpura. Methods The expression of T-bet, GATA-3 and RORC mRNA in 32 peripheral blood mononuclear cells of allergic purpura and 15 normal controls were detected by SYBR GreenI real-time PCR. Results The mean ± SE values of T-bet gene in patients with allergic purpura were 27.70 and 16.10, respectively. The expression level of T-bet mRNA in patients with allergic purpura was lower than that in controls. The average ΔCt of GATA-3 gene in patients with allergic purpura (18.25 and 36.27, respectively). The expression of GATA-3 mRNA in patients was higher than that in controls. The ΔCt values of RORC gene in patients with allergic purpura and normal subjects were 2.00 ± 0.46 and 3.29 ± 1.57, respectively. The RORC mRNA expression in patients with allergic purpura was higher than that in controls Group, the differences were statistically significant (P all <0.05). Conclusion The transcription factors T-bet, GATA-3 and RORC may play an important role in the pathogenesis of Henoch-Schonlein purpura.