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目的了解肺泡表面活性物质蛋白B(SPB)-18A/C和SPB1580C/T基因多态性与支气管肺发育不良(BPD)易感性的关系。方法应用聚合酶链反应-限制性片段长度多态分析技术及基因测序技术检测57例BPD患儿及103例同胎龄无BPD早产儿的SPB-18及SPB1580基因多态性,比较2组SPB-18及SPB1580基因型和基因分布的差异。结果 1.SPB-18A/C基因型在BPD组与对照组AA、AC、CC基因型频率分别为14.0%、45.6%、40.4%和4.9%、31.1%、64.1%;A等位基因增加新生儿患BPD的风险:AC/CC与AA/CC的OR值分别为2.3(95%CI1.2～4.7,P=0.02)和4.7(95%CI1.4～16.2,P=0.01);2.SPB1580C/T基因型在对照组和BPD组TT、CT、TT分布分别为6.8%、45.6%、47.6%和12.3%、40.4%、47.4%,对照组和BPD组比较差异无统计学意义(χ2=1.50,P>0.05)。结论中国武汉汉族人中SPB-18基因多态性是BPD的危险因素,基因型为AA者是BPD易感人群。SPB1580C/T基因多态性与BPD发病无明显相关性。
Objective To investigate the relationship between the polymorphisms of alveolar surfactant protein B (SPB) -18A / C and SPB1580C / T and the susceptibility to bronchopulmonary dysplasia (BPD). Methods The polymorphisms of SPB-18 and SPB1580 in 57 children with BPD and 103 children without BPD with gestational age were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing. The SPB- -18 and SPB1580 genotypes and gene distribution differences. The genotype frequencies of AA, AC and CC of the SPB-18A / C genotype in the BPD group and the control group were 14.0%, 45.6%, 40.4% and 4.9%, 31.1% and 64.1% respectively; the A allele increased Risk of BPD: The odds ratios for AC / CC and AA / CC were 2.3 (95% CI 1.2 to 4.7, P = 0.02) and 4.7 (95% CI 1.4 to 16.2, P = 0.01), respectively; The distribution of TT, CT and TT of SPB1580C / T genotype in control group and BPD group were 6.8%, 45.6%, 47.6% and 12.3%, 40.4% and 47.4%, respectively. There was no significant difference between control group and BPD group (χ2 = 1.50, P> 0.05). Conclusion SPB-18 gene polymorphism is a risk factor for BPD in Wuhan Han Chinese and genotype AA is BPD susceptible. There was no significant correlation between SPB1580C / T polymorphism and BPD.