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目的 观察脊髓灰质细胞的分化发育 ,寻找脊髓移植时胎鼠脊髓取材的最佳时间。 方法 用 5 -溴 -2 -脱氧尿嘧啶 (Brd U)标记 DNA,ABC免疫细胞化学方法观察大鼠上胸段脊髓灰质细胞的增殖分化情况。 结果孕鼠于 E10 d腹腔注射 Brd U,胎鼠脊髓上胸段 Brd U免疫反应神经元主要集中在脊髓后角 ,脊髓侧角的 Brd U免疫反应神经元较少 ,前角仅有少量的 Brd U免疫反应神经元 ;孕鼠于 E11d腹腔注射 Brd U,胎鼠脊髓上胸段后角 Br-d U免疫反应神经元较 E10 d减少 ,侧角和前角 Brd U免疫反应神经元增多 ;孕鼠于 E12 d腹腔注射 Brd U,胎鼠脊髓上胸段 Brd U免疫反应神经元主要集中于前角和侧角 ,而后角仅有少量的 Brd U免疫反应神经元 ;孕鼠于 E13d、E14d腹腔注射 Brd U,胎鼠脊髓上胸段未见 Brd U免疫反应神经元。 结论 Brd U标记技术能较好的标记脊髓灰质细胞 ,胎鼠脊髓进行 Brd U标记的最佳时间应选择 E12 d,已标记胎鼠脊髓的最佳移植取材时间为 E13d
Objective To observe the differentiation and development of the spinal cord gray matter cells and to find out the best time to draw the spinal cord of fetal rat during spinal cord transplantation. Methods The proliferation and differentiation of the upper thoracic spinal cord gray matter in rats were observed by using BrdU labeled DNA and ABC immunocytochemistry. Results BrdU was injected intraperitoneally on E10 d of pregnant rats. The BrdU immunoreactive neurons in the upper thoracic spinal cord of pregnant rats mainly concentrated in the posterior horn of spinal cord. There were fewer BrdU immunoreactive neurons in the lateral horn of spinal cord and only a small amount of Brd U immunoreactive neurons. BrdU was injected intraperitoneally in E11d pregnant rats, the Br-d U immunoreactive neurons in the thoracic antrum of the fetal rat decreased compared with those in E10 d, and the number of BrdU immunoreactive neurons in lateral horn and anterior horn increased BrdU was intraperitoneally injected on E12 mice. BrdU immunoreactive neurons in the upper thoracic spinal cord of fetal rats mainly focused on the anterior horn and lateral horn, while only a small amount of BrdU immunoreactive neurons were observed in the posterior horn. E14d and E14d celiac There was no BrdU immunoreactive neurons in the upper thoracic spinal cord of BrdU injected BrdU. Conclusion The BrdU labeling technique can be used to label the spinal cord gray matter cells. The optimal time for BrdU labeling in fetal rat spinal cord should be E12 d. The optimal transplantation time for labeled fetal rat spinal cord is E13d