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目的探讨表皮生长因子(EGF)促细胞增殖的剂量-效应关系及对萎缩性胃炎(CAG)大鼠胃黏膜增生作用的影响。方法用不同浓度EGF对培养的人羊膜细胞进行不同时间段刺激, MTT法和蛋白印迹法(Western blot)检测细胞增殖情况及细胞外信号调节蛋白激酶(Erk1／2)变化结果;建立CAG大鼠模型,对CAG大鼠皮下注射EGF,12周后观察大鼠胃黏膜病理变化。结果剂量-时间-效应关系示EGF浓度10μg／L第3天时促细胞增殖率最高(42．4％,P<0．01),Western blot结果示EGF浓度10～60μg／L时磷酸化Erk1／2活性显著升高;动物实验示EGF治疗组胃黏膜腺体排列整齐规则,炎性细胞极少,明显好于对照组,治疗组(rhEGF和羊EGF)胃黏膜增殖细胞核抗原(PCNA)表达阳性的宽度分别为(77．70±4．16)μm和(75．60±2．92)μm,较对照组(54．40±4．54)μm明显增加(P<0．01)。结论EGF促细胞增殖及激活Erk1／2通路有明显的剂量或时间依赖性作用,EGF对SD大鼠萎缩性胃黏膜有保护性促增生作用。
Objective To investigate the dose-response relationship between epidermal growth factor (EGF) cell proliferation and gastric mucosal hyperplasia in rats with atrophic gastritis (CAG). Methods The cultured human amniotic cells were stimulated with different concentrations of EGF for different time periods. The cell proliferation and the expression of extracellular signal-regulated protein kinase (Erk1 / 2) were detected by MTT assay and Western blotting. CAG rats Model, CAG rats were injected subcutaneously with EGF, and the pathological changes of gastric mucosa were observed 12 weeks later. Results The dose-time-effect relationship showed that the cell proliferation rate was the highest (42.4%, P <0.01) on the 3rd day when the EGF concentration was 10μg / L. The results of Western blot showed that the phosphorylation of Erk1 / 2 activity was significantly increased; animal experiments showed that gastric mucosal glands in EGF treatment group neat rules, very few inflammatory cells, was significantly better than the control group, the treatment group (rhEGF and sheep EGF) positive expression of gastric mucosal proliferating cell nuclear antigen (PCNA) (77.70 ± 4.16) μm and (75.60 ± 2.92) μm respectively, which was significantly higher than that of the control group (54.40 ± 4.54) μm (P <0.01). Conclusion EGF promoted the proliferation and activated Erk1 / 2 pathway in a dose-and time-dependent manner. EGF had a protective effect on atrophic gastric mucosa of SD rats.