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目的 了解Epstein Barr(EB)病毒基因bhrfl表达对鼻咽癌细胞拓扑异构酶Ⅰ表达的影响。方法 通过构建bhrfl表达载体并转染鼻咽癌细胞株CNE2细胞 ,以检测癌细胞在转染后其拓扑异构酶Ⅰ表达及其抗喜树碱诱发凋亡能力的改变。结果 bhrfl表达可增强癌细胞拓扑异构酶Ⅰ的表达 ,使癌细胞对喜树碱的敏感性下降 ,生存时间延长 ,凋亡率减少 (P <0 0 1)。结论 bhrfl的表达可通过增强CNE2细胞拓扑异构酶Ⅰ的表达而增强其抗喜树碱诱发的细胞凋亡作用。
Objective To investigate the effect of bhrfl expression of Epstein Barr (EB) gene on the expression of topoisomerase Ⅰ in nasopharyngeal carcinoma cells. Methods The bhrfl expression vector was transfected into nasopharyngeal carcinoma cell line CNE2 to detect the expression of topoisomerase Ⅰ and its anti-camptothecin-induced apoptosis after transfection. Results bhrfl expression enhanced the expression of topoisomerase Ⅰ in cancer cells, decreased the sensitivity of cancer cells to camptothecin, prolonged survival and reduced the apoptosis rate (P <0.01). Conclusion The expression of bhrfl enhances the anti-camptothecin-induced apoptosis in CNE2 cells by enhancing the expression of topoisomerase Ⅰ.