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[目的]通过对月月红中槲皮素、山奈酚的定性定量研究,拟建立其HPLC分析方法,为进一步研究民族民间药物月月红的药效学奠定基础。[方法]采用化学定性法确定月月红不同部位中的主要化学组分;色谱条件:Hypersil DJZ C18色谱柱(4.6 mm×200.0 mm,5μm);流动相:甲醇-0.4%磷酸(60∶40,V/V);检测波长:368 nm;流速:1.000 ml/min;柱温:30℃;理论塔板数按山奈酚和槲皮素峰计算不低于3 000,外标法计算。[结果]槲皮素在0.081 6~0.489 6μg线性关系良好(R2=0.999 6),平均回收率为101.6%,RSD为0.87%;山奈酚在0.069 6~0.417 6μg线性关系良好(R2=0.999 6),平均回收率为96.8%,RSD为1.01%。月月红叶中槲皮素为0.038 2%,茎为0.005 2%,全株为0.010 5%;月月红叶中山奈酚含量为0.027 6%,茎为0.002 1%,全株为0.009 6%。[结论]HPLC分析方法稳定、重复性较好;月月红不同部位均含有槲皮素、山奈酚,且叶含量高于全株和茎。
[Objective] To establish a method for the determination of quercetin and kaempferol in moon and moon red by HPLC, and lay the foundation for the further study on the pharmacodynamics of moon and moon red, a folk medicine. [Method] The main chemical components in different parts of Moon Moon Red were determined by chemical qualitative method. The chromatographic conditions were Hypersil DJZ C18 column (4.6 mm × 200.0 mm, 5 μm); mobile phase: methanol -0.4% phosphoric acid (60:40 , V / V). The detection wavelength was 368 nm. The flow rate was 1.000 ml / min. The column temperature was 30 ℃. The number of theoretical plates was calculated to be not less than 3,000 in terms of kaempferol and quercetin peaks. [Result] The calibration curve of quercetin was linear in the range of 0.081 6 ~ 0.489 6μg (R2 = 0.999 6). The average recovery was 101.6% and the RSD was 0.87%. The linear relationship of kaempferol at 0.069 6 ~ 0.417 6μg was good (R2 = 0.999 6 ), The average recovery was 96.8%, RSD was 1.01%. There were 0.038 2% quercetin, 0.005% 2% stems and 0.010 5% stems in the autumn leaves. The content of nematan in the leaves of month-month was 0.027 6%, 0.002 1% for stems and 0.009 6% for whole plants. [Conclusion] The method of HPLC analysis was stable and reproducible. Quercetin and kaempferol were contained in different parts of Moon Moon Red, and the contents of leaf were higher than those of whole plant and stem.