人肝细胞的培养,由于它既可为肝癌细胞的研究作出实验对比,又将为肝病的病因探讨(如化学致癌物质、肝炎病毒等的致癌机理)提供近似人体的实验模型,因而越来越受到人们的关注。据了解,目前在国内外尚未有人体正常肝细胞巴林成功建报导。1977年11月25日,我们从一例猝死尸解标本(男、47岁)获得正常肝组织(经病理切片证实),进行了体外培养。目前,细胞生长良好,历时17个月,传了60多代,定名为QZG—7702。现将核细胞的培养过程及其生物学特征上的变异简要介绍于下。细胞培养过程以无菌操作取得肝组织,进行离体培养。培养10天后陆续有成纤维细胞长出,随之又逐渐出现上皮型细胞。一个多月后,生长晕的细胞茂密,以上皮型细胞为主,此时,即行传代。第一代细胞就能较好地贴壁生长。当传至第3～5代时,出现了近一个月的缓慢生长期,通过适当的处理后,第6代细胞的生长、繁殖又恢复正常。此时,成纤维细胞逐渐退化消失,上皮型细胞生长占优势。 Human hepatocyte culture, because it can make experiments and comparisons for the study of liver cancer cells, and it will provide an experimental model for the etiology of liver diseases (such as chemical carcinogens, hepatitis viruses, and other carcinogenic mechanisms) to approximate the human body, and thus more and more Concerned by people. It is understood that Bahrain, a normal human liver cell, has not been successfully reported at home and abroad. On November 25, 1977, we obtained normal liver tissue (observed by pathological section) from a case of dead deceased man (male, 47 years old) and cultured in vitro. At present, the cells have grown well and lasted for 17 months and passed over 60 generations. The name was QZG-7702. The nucleus cell culture process and its biological characteristics are briefly introduced below. During the cell culture process, liver tissue was obtained aseptically and cultured in vitro. Ten days after culture, fibroblasts were gradually formed, and epithelial cells gradually emerged. After more than one month, the cells growing halo are dense and the epithelial cells are predominant. At this time, the cells are passaged. The first generation of cells will grow well on the wall. When passed to the 3rd to 5th generations, a slow growth period of nearly one month occurred. After proper treatment, the growth and reproduction of the 6th generation cells returned to normal. At this point, fibroblasts gradually degenerate and disappear, and epithelial cells grow predominantly.