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目的:建立液相色谱-串联质谱法(LC-MS/MS)同时测定家禽和家畜肉中8种磺胺类药物(磺胺二甲氧基嘧啶、磺胺二甲嘧啶、磺胺甲基嘧啶、磺胺甲氧嘧啶、磺胺甲基异噁唑、磺胺吡啶、磺胺二甲异嘧啶、磺胺异噁唑)残留量。方法:以依普沙坦为内标,肉类样品匀浆后经乙腈沉淀提取,提取液加水稀释后,采用反相色谱法分离、电喷雾正离子模式下多反应监测定量分析。结果:线性范围为10～300ng·g-1(r>0.99),最低定量限为10ng·g-1(S/N≥10),准确度为86.0%～108.9%;批内和批间精密度均小于10%。结论:本方法操作简便,特异性强,灵敏度高,取样量少,符合生物样品的分析要求,可以用于肉类食品中的磺胺类药物多残留分析。
OBJECTIVE: To establish a liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for the simultaneous determination of 8 sulfonamides in poultry and livestock meat (sulfamethoxypyrimidine, sulfamethazine, sulfamerazine, Pyrimidine, sulfamethoxazole, sulfapyridine, sulfamethazine, sulfisoxazole) residues. Methods: Epulsartan was used as an internal standard. Meat samples were homogenized and then extracted with acetonitrile. The extracts were diluted with water and separated by reversed-phase chromatography. The multi-reaction monitoring and quantification was performed in electrospray positive ion mode. Results: The linear range was 10 ~ 300 ng · g-1 (r> 0.99), the lowest limit of quantification was 10 ng · g-1 (S / N≥10), and the accuracy was 86.0% -108.9% Degrees are less than 10%. Conclusion: The method is simple, specific, sensitive, sampling less, in line with the analysis of biological samples, can be used for meat sulfonamides multi-residue analysis.