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以ＰＣＲ产物为测序模板，γ３２ＰＡＴＰ标记的ＰＣＲ扩增引物为测序引物，用ＴａｑＤＮＡ聚合酶为测序酶，建立了庚型肝炎病毒（ＨＧＶ）ｃＤＮＡ序列分析方法。应用本法测定１份ＰＣＲ阳性产物的ＨＧＶｃＤＮＡ序列，图谱呈清晰的序列梯，其序列与ＧＢＶＣ（Ｕ３６３８０）和ＨＧＶ（Ｕ４４４０２）的相应片段序列比较，同源性分别为８５６５％（１９１／２２３）和８５２％（１９０／２２３）。与用荧光法测定的另一株ＨＧＶ序列有高度一致性。对同一样品两端测序，重叠部分序列完全一致。表明ＰＣＲ产物直接测序法可用于ＨＧＶ核酸序列分析。
PCR products as the sequencing template, γ 32P ATP-labeled PCR amplification primers for sequencing primers, using Taq DNA polymerase as a sequencing enzyme, the establishment of a hepatitis G virus (HGV) cDNA sequence analysis. The HGV cDNA sequence of 1 PCR positive product was determined by this method. The sequence of the HGV cDNA was a clear ladder. The homology was 8565% (corresponding to the corresponding fragment of GBVC (U36380) and HGV (U44402)) 191/223) and 85 2% (190/223). Highly consistent with another HGV sequence determined by fluorometry. Both ends of the same sample sequencing, overlapping part of the sequence is exactly the same. The PCR product direct sequencing method can be used for HGV nucleic acid sequence analysis.