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目的:比较溶血空斑试验(PFC)和微量溶血分光光度法(QHS)检测免疫低下小鼠抗体形成细胞功能的优缺点。方法:BALB/C种小鼠用环磷酰胺(CP)造成免疫低下,分成四个80 mg/kg CP组和四个60 mg/kg CP组,其下均设模型对照组及蛹虫草子实体高、中、低三个剂量组。另设正常小鼠组。30 d后进行PFC和微量QHS。另外用80 mg/kg CP模型对照组及60 mg/kg CP模型对照组小鼠不同脾细胞浓度做微量QHS。结果:环磷酰胺80 mg/kg BW和60 mg/kg BW制造免疫低下小鼠模型成立,蛹虫草子实体高、中、低三个剂量组溶血空斑数和吸光度与CP模型对照组比较差异均无显著性意义,两种检测方法所得的结果一致,且溶血空斑数与吸光度值呈正相关。PFC法显示免疫低下80 mg/kg CP模型对照组和60 mg/kg CP模型对照组小鼠的溶血空斑数仅为正常小鼠的11%和12%,而微量QHS测得的A值则为正常小鼠组的55%和58%,表明两种检测方法存在差异。本研究为明确这一差异的产生原因,对CP模型对照组不同脾细胞浓度进行微量QHS检测,结果显示脾细胞数在1×107-4×107之间时与A值线性关系良好。结论:两种方法均能有效地检测抗体形成细胞的功能,但在抗体形成细胞功能组间有很大差异时,PFC能更直观准确的表现其差异,较微量QHS法优越。
OBJECTIVE: To compare the advantages and disadvantages of using hemolytic plaque assay (PFC) and microhemolytic spectrophotometry (QHS) to detect the function of antibody-forming cells in immunocompromised mice. Methods: BALB / C mice were immunized with cyclophosphamide (CP) and divided into four groups of 80 mg / kg CP and four groups of 60 mg / kg CP. The model control group and Cordyceps militaris High, medium and low dose of three groups. Another set of normal mice group. After 30 days PFC and trace QHS were performed. In addition, 80 mg / kg CP model control group and 60 mg / kg CP model control group of mice with different concentrations of spleen cells to do a small amount of QHS. Results: Immunosuppressive mice model was established by cyclophosphamide at 80 mg / kg BW and 60 mg / kg BW. The numbers of hemolytic plaque and the absorbance in the high, middle and low dose groups of Cordyceps militaris were significantly lower than those of the CP model control group No significant significance, the results obtained by the two detection methods consistent, and the hemolytic plaque number and absorbance values were positively correlated. PFC method showed that the immunosuppressive plaque number of the immunocompromised 80 mg / kg CP model control group and the 60 mg / kg CP model control group mice was only 11% and 12% of that of the normal mice, while the trace QHS measured A value 55% and 58% of the normal mouse group, indicating that there is a difference between the two detection methods. This study to clarify the causes of this difference, the CP model control group of different concentrations of spleen cells were detected by QHS, the results showed that when the number of spleen cells between 1 × 107-4 × 107 A value and good linear relationship. CONCLUSION: Both of these methods can effectively detect the function of antibody-forming cells. However, when there is a great difference between the functional groups of antibody-forming cells, PFC can show the difference more directly and accurately than the QHS method.