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以甜瓜(Cucumis melo L.)黄绿皮、白肉、无覆纹的自交系‘K1-7’为母本,黄皮、橘红肉、无覆纹的自交系‘K3-92-1’为父本杂交,组建F2遗传群体,采用MSG(multiplexed shotgun genotyping)法对F2群体进行基因分型,并在甜瓜全基因组范围内扫描SNP遗传标记。利用检测到的44 722个SNP标记位点进行高密度Bin遗传图谱的构建,并对甜瓜果皮底色、覆纹颜色和果肉颜色等3个质量性状进行遗传分析和基因定位。结果表明,果皮底色和果肉颜色由单基因控制,黄绿果皮和橘红色果肉为显性性状,果皮底色基因定位在4号染色体409 828～835 625 bp区间内,长度约为425 kb,果肉颜色基因定位在9号染色体20 433 942～20 573 889 bp区间内,长度约为139 kb,覆纹颜色由两个有上位效应的基因控制,其中1个基因位点与果皮颜色控制位点在同一区域,1个基因定位在2号染色体末端23 736 803～23 787 235 bp区间内,长度约为51 kb。
The inbred lines ’K3-92-1’ with yellow-green skin, white meat and non-covered lines were used as female parent, yellow skin, orange-red meat, The F2 population was crossed with the male parent. The F2 population was genotyped by multiplexed shotgun genotyping (MSG) and the SNP was detected in the whole genome of melon. The genetic map of high-density Bin was constructed using 44 722 SNP markers detected, and the genetic analysis and gene mapping were conducted on three quality traits, peel color, coat color and flesh color. The results showed that the color of the peel and the color of the flesh were controlled by a single gene, and the yellow-green peel and the tangerine flesh were dominated by the dominant color. The base color of the peel was located in the range of 409 828-835 625 bp on chromosome 4, The color of the flesh chromosome was located in the interval of 20 433 942 ~ 20 573 889 bp on chromosome 9 with a length of about 139 kb. The color of the flock was controlled by two epistatic genes. One locus was related to the color control site In the same region, a gene locates at 23 736 803 ~ 23 787 235 bp at the end of chromosome 2 and has a length of about 51 kb.