人胚胎干细胞(hESCs)不仅为基础研究提供了独特的模型,而且在细胞移植治疗方面有可能存在巨大的应用前景。近年来,hESCs培养体系的优化已取得了巨大进展,研究者们通过使用机械法切割内细胞团(ICM),以及在培养过程中使用无动物成分的培养液、饲养层或基质胶,或使用无饲养层培养体系并且维持染色体核型的稳定性等,以得到有质量和安全保证、可用于临床治疗的干细胞。 Human embryonic stem cells (hESCs) not only provide a unique model for basic research, but also have potential applications in cell transplantation. In recent years, tremendous advances have been made in the optimization of culture systems for hESCs. Researchers have used mechanical methods to cut inner cell masses (ICMs), as well as the use of animal-free media, feeder layers or matrigel during culture, or the use of Feeder-free culture system and maintain the stability of the chromosome karyotype, etc., in order to get quality and safety assurance, can be used for clinical treatment of stem cells.