目的了解深圳口岸出入境人员流感病毒感染的流行病学特征。方法选择深圳福田地铁、深圳湾和罗湖口岸3个国境口岸为出入境流感监测点,对监测到的流感样病例采集咽拭子标本,并进行流行病学调查。运用荧光实时RT-PCR技术检测流感样病例的咽拭子标本,结合流行病学资料进行分析。结果2008年7月～2009年2月,在3个深圳口岸监测到的流感样病例为441份,其中,55份流感样病例的咽拭子标本中流感病毒核酸检测阳性,总检出率为12.5%。其中A型流感病毒核酸阳性47例,占85.5%,B型流感病毒核酸阳性8例,占14.5%;男性41例,占74.5%,女性11例,占20.0%,性别不详者3例,占5.5%;儿童(15岁以下)23例,占41.8%,成人32例,占58.2%。结论2008～2009年深圳口岸出入境人员流感样病例中以A型流感病毒感染为主,了解深圳国境口岸出入境人员流感病毒感染的情况,预防和控制出入境人员流感病毒的流行具有十分重大的意义。 Objective To understand the epidemiological characteristics of influenza virus infection in entry-exit personnel in Shenzhen Port. Methods Three border crossings of Shenzhen Futian Subway, Shenzhen Bay and Lo Wu Ports were selected as surveillance points for influenza inbound and outbound. Throat swab samples were collected from the monitored influenza-like cases and epidemiological investigation was conducted. Fluorescent real-time RT-PCR was used to detect the throat swab samples of influenza-like cases and analyzed with epidemiological data. Results From July 2008 to February 2009, 441 flu-like cases were detected in three Shenzhen ports, of which 55 were positive for influenza virus nucleic acid in throat swab samples, the total detection rate was 12.5%. Among them, 47 were positive for type A influenza virus (85.5%), 8 were positive for type B influenza virus (14.5%), 41 were male (74.5%), 11 were female (20.0%), 3 were gender unknown 5.5%; children (under 15 years old) 23 cases, accounting for 41.8%, 32 adults, accounting for 58.2%. Conclusions Influenza A virus infection is the main source of influenza-like illness among immigrants arriving at Shenzhen Port from 2008 to 2009. It is very important to know the prevalence of influenza virus among immigrants at the border crossings in Shenzhen. significance.