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目的探讨模拟失重对成骨细胞力学信号转导功能的影响。方法体外传代培养的大鼠乳鼠颅骨成骨细胞分为两组 ,一组置于回转器中培养 ,另一组则在地面重力环境下培养。 60h后 ,将成骨细胞置于流室系统中进行刺激实验。流体剪切应力 (FSS)分别为 0 .5Pa和 1 .5Pa ,作用时间分别为 30min和60min。其后进行免疫组化染色和图像分析。结果在 1G组 ,相同时间不同水平FSS作用诱导的环氧合酶 - 2 (COX 2 )蛋白表达无显著差异 ,FSS作用 30min与 60min所诱导的COX 2蛋白表达差异有显著性意义 (P <0 .0 1 ) ;模拟失重环境下COX 2蛋白表达发生下调性变化 ,仅在 1 .5PaFSS作用 60min的成骨细胞中检测到蛋白表达。相同时间和FSS下 ,模拟失重组与 1G组COX 2蛋白表达水平的差异有显著性意义 (P <0 .0 1 )。结论模拟失重时成骨细胞的力学信号转导功能发生了显著的下调性改变
Objective To investigate the effect of simulated weightlessness on mechanical signal transduction in osteoblasts. Methods The rat osteoblasts cultured in vitro were divided into two groups. One group was placed in a rotator and the other group was cultured in the ground gravity environment. After 60 h, osteoblasts were placed in a flow chamber system for stimulation experiments. Fluid shear stress (FSS) were 0. 5Pa and 1 .5Pa, the action time was 30min and 60min. Subsequent immunohistochemical staining and image analysis. Results There was no significant difference in the expression of cyclooxygenase - 2 (COX - 2) induced by FSS at different time points in the 1G group. The COX 2 protein expression induced by FSS for 30 min and 60 min was significantly different (P <0. .0 1). Under the simulated weightlessness environment, the expression of COX-2 protein was down-regulated. The expression of COX-2 protein was only detected in osteoblasts treated with 1.5PaFSS for 60min. At the same time and under FSS, there was a significant difference in COX2 protein expression between simulated weightlessness group and 1G group (P <0.01). Conclusions There is a significant down-regulation of mechanical signal transduction in osteoblasts under simulated weightlessness