论文部分内容阅读
采用液体深层发酵方式,研究了几种药用昆虫对灵芝多糖生物合成的影响。结果表明,药用昆虫蜣螂在添加量为5g/L时能显著促进灵芝胞内多糖(IPS)和胞外多糖(EPS)的形成(P<0.05)。胞内多糖和胞外多糖的产量分别由对照的(1.93±0.09)g/L和(520.3±20.2)mg/L提高到(2.41±0.12)g/L和(608.9±20.2)mg/L。灵芝胞内多糖和胞外多糖在DEAE纤维素柱上都可分离得到5种主要组分,其中IPS-1和EPS-1分别为2类多糖的主要组分。进一步用凝胶柱分离显示,IPS-1由3个单个的组分组成,EPS-1由2个单个的组分组成。添加蜣螂发酵后,灵芝胞内多糖和胞外多糖中没有出现新的组分,且各组分的相对含量也没有显著变化(P>0.05),提示添加蜣螂发酵后,灵芝胞内多糖和胞外多糖主要组分的合成途径并未改变。
The effects of several medicinal insects on the biosynthesis of Ganoderma lucidum polysaccharides were studied by liquid submerged fermentation. The results showed that medicinal insects dung beetle could significantly promote the formation of polysaccharide (EPS) and extracellular polysaccharides (EPS) in Ganoderma lucidum at the dosage of 5g / L (P <0.05). The production of intracellular polysaccharides and extracellular polysaccharides increased from (1.93 ± 0.09) g / L and (520.3 ± 20.2) mg / L to (2.41 ± 0.12) g / L and (608.9 ± 20.2) mg / L, respectively. Ganoderma lucidum intracellular polysaccharides and exopolysaccharides in DEAE cellulose column can be isolated from five main components, of which IPS-1 and EPS-1 were two kinds of polysaccharides, the main component. Further separation by gel column showed that IPS-1 consisted of 3 individual components and EPS-1 consisted of 2 individual components. After the addition of dung beet ferment, no new components appeared in the polysaccharides and exopolysaccharides of Ganoderma lucidum, and the relative contents of the components did not change significantly (P> 0.05), which indicated that after adding the dung beet ferment, the Ganoderma lucidum intracellular polysaccharides And the main component of the extracellular polysaccharide synthesis pathway has not changed.