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目的分析苏黄止咳胶囊及其挥发油中间体中的挥发性成分。方法水蒸气蒸馏法提取挥发油后,GC-MS结合保留指数法分析其成分,峰面积归一化法计算相对含有量,再通过聚类分析和主成分分析考察6批样品(胶囊、中间体各3批)之间的差异。结果从3批胶囊中检测出119个色谱峰,并鉴定出54种成分,分别占挥发油总量的53.80%、50.77%、46.66%;从3批中间体中检测出74个色谱峰,并鉴定出48种成分,分别占挥发油总量的85.14%、86.28%、85.41%。不同批次胶囊和中间体均能得到较好的分类,并且γ-松油烯、异松油烯、松油-4-醇、α-松油醇4种单萜是影响聚类结果的主要成分。结论该方法可阐明苏黄止咳胶囊及其挥发油中间体中挥发性成分组成及其含有量的差异,为两者质量控制提供科学依据。
Objective To analyze the volatile components in the capsule of Suhuangzhike and its volatile oil intermediates. Methods After volatile oil was extracted by steam distillation, the components were determined by GC-MS and retention index method, and the relative content was calculated by the normalization of peak area. Then, six batches of samples (capsules and intermediates 3 batches) between the differences. Results 119 chromatographic peaks were detected from 3 batches of capsules and 54 components were identified, accounting for 53.80%, 50.77% and 46.66% of the total volatile oil respectively. 74 peaks were detected from 3 batches of intermediates and identified Out of 48 kinds of ingredients, accounting for 85.14% of the total volatile oil, 86.28% and 85.41% respectively. Different batches of capsules and intermediates can be better classified, and γ-terpinene, terpinolene, terpineol-4-ol, α-terpineol 4 kinds of monoterpenes are the main impact of clustering results ingredient. Conclusion This method can clarify the volatile constituents and the content of volatile components in Suhuangzhike Capsule and its volatile oil intermediates, and provide a scientific basis for the quality control of both.