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HiCN法测定血红蛋白(HGB)是国际血液学标准化委员会推荐的参考方法。其测定原理是:HGB被高铁氰化钾氧化成高铁血红蛋白后,再与氰离子结合形成稳定的氰化高铁血红蛋白(HiCN),在540nm波长处HiCN具有特异的吸收峰,可用分光光度计进行测定[1]。但当标本量较大时,用分光光度计比色却
HiCN method for the determination of hemoglobin (HGB) is recommended by the International Commission of Hematology standard reference method. The determination principle is: HGB was ferricyanide into methemoglobin, and then combined with cyanide to form stable cyanide methemoglobin (HiCN), HiCN at 540nm wavelength has a specific absorption peak can be measured spectrophotometer [1]. However, when the specimen size is larger, the colorimetric is measured with a spectrophotometer