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利用PCR技术获得了甘薯茎线虫rDNA-ITS1区序列。序列分析表明,采自我国河北、山东、安徽的甘薯茎线虫16个地理种群的ITS1区序列分化为短型(S)和长型(L)2种基因型。山东费县芍药山乡4个地理种群为L型,ITS1区长度为466 bp;河北、安徽和山东费县新庄乡的12个地理种群为S型,ITS1区长度为288 bp。甘薯茎线虫与鳞球茎茎线虫(Di-tylenchus dipsaci)的rDNA-ITS1序列同源性为52.0%~52.5%,与腐烂茎线虫(D.destructor)序列同源性为82.0%~85.4%;我国甘薯茎线虫不同地理种群间的序列同源性为96.6%~100.0%。
The rDNA-ITS1 region of S. dipsaci was obtained by PCR. Sequence analysis showed that ITS1 sequences of 16 geographic populations of D.sinensis from Hebei, Shandong and Anhui Provinces differentiated into two genotypes, short (S) and long (L). Four geographical populations in Shaofu Mountain, Feixian County, Shandong Province, are L type and the length of ITS1 region is 466 bp. The 12 geographic populations of Xinzhuang Township in Hebei Province, Anhui Province and Fei County of Shandong Province are S type and ITS1 region is 288 bp in length. The sequences of rDNA-ITS1 shared 52.0% -52.5% identity with D-tylenchus dipsaci and 82.0% -85.4% with D. destructor. Our country The sequence homology among different geographical populations of D. solani was 96.6% -100.0%.