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本研究从豚鼠脑组织中提取微管蛋白,利用微管蛋白在不同温度或药物的作用下,能发生聚合或解聚的特点,借助紫外分光光度分析和电镜观察,在体外条件下研究治疗矽肺药物的汉防己甲素(简称汉甲素)对微管蛋白的作用,并以秋水仙硷作对照药物。汉甲素对微管蛋白或微管的作用:当剂量在0.1mM时,无明显的微管毒作用;当剂量增加一倍以上或延长作用时间后,汉甲素能使微管凝聚,最后导致微管破坏,表现与秋水仙硷有类似的作用,扰乱了前胶原从细胞中向外分泌,这可能与汉甲素抑制矽肺胶原纤维化有关。
In this study, we extracted tubulin from the brain tissue of guinea pigs and used the ability of tubulin to polymerize or depolymerize under the action of different temperature or drugs. Using UV spectrophotometry and electron microscopy to study the treatment of silicosis Drugs of tetrandrine (abbreviated to Chinese A) on tubulin, and colchicine as a control drug. Norepinephrine on tubulin or microtubules: When the dose of 0.1mM, no significant microtubule toxicity; when the dose more than doubled or extended the action time, the Chinese tuberculin can make microtubules cohesion, and finally Lead to the destruction of microtubules, the performance of colchicine have a similar role, disrupt the secretion of procollagen from the cells, which may be related to the inhibition of melatonin in silicotic collagen fibrosis.