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目的 了解儿童血液病人细小病毒B19(HPVB19)感染的状况及其关系。方法 应用巢式聚合酶链反应检测 90例儿童血液病患者 [其中再生障碍性贫血 (AA) 4 0例 ,特发性血小板减少性紫癜 (ITP) 2 4例 ,急性淋巴细胞性白血病 (ALL) 18例 ,难治性贫血 (RA) 8例 ]血清或骨髓中HPVB19-DNA ,并以 30例健康儿童为对照。结果 病例组HPVB19-DNA的总阳性检出率为 2 8 9% (2 6 / 90 ) ,其中AA、ITP、ALL及RA患儿中HPVB19-DNA的阳性率分别为 :2 5 0 %、2 9 2 %、33 3 %和37 5 % ,与对照组比较 ,均有显著性差异 (P <0 0 1或P <0 0 5 )。结论 HPVB19感染可能与儿童AA和ITP的发病有关 ;接受化疗的ALL患儿易继发HPVB19感染 ,并可致骨髓抑制和慢性贫血
Objective To investigate the status of children’s blood parvovirus B19 (HPVB19) infection and its relationship. Methods Nested polymerase chain reaction was used to detect 90 cases of hematological diseases in children (including 40 cases of aplastic anemia (AA), 24 cases of idiopathic thrombocytopenic purpura (ITP), 24 cases of acute lymphoblastic leukemia (ALL) 18 cases, 8 cases of refractory anemia (RA)] serum or bone marrow HPVB19-DNA, and 30 healthy children as a control. Results The positive rate of HPVB19-DNA in cases was 29.8% (26/90). The positive rates of HPV-B19-DNA in AA, ITP, ALL and RA were 25.0% and 2% respectively 9 2%, 33 3% and 37 5%, respectively, which were significantly different from the control group (P <0.01 or P <0.05). Conclusion The infection of HPVB19 may be related to the pathogenesis of AA and ITP in children. ALL patients receiving chemotherapy are prone to be infected with HPVB19 and may cause myelosuppression and chronic anemia