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短丝木犀(Osmanthus serrulatus)是我国特有的木犀属香花树种,具有极高的开发应用前景。笔者以短丝木犀为研究材料,应用Illumina HISeqTM2000高通量测序平台,对短丝木犀的花和叶芽进行转录组深度测序和拼接组装,构建和预测了短丝木犀不同组织间的基因表达谱及其代谢通路。转录组测序共获得16.94 G的有效数据,经Trinity从头组装得到92 798条单基因簇(unigenes),其中35 851条unigenes与Nr、Nt、Pfam、KOG、Swiss-Prot、KEGG、PFAM等7大公共数据库比对获得了基因功能注释信息。由KEGG代谢通路分析发现,共有8 779条unigenes参与到262个代谢通路中,其中参与到色素和香味代谢的基因分别有53条和335条。此外,针对6条在短丝木犀花和叶芽间显著差异表达的参与类胡萝卜素生物合成的相关基因,通过实时荧光定量PCR,验证了它们在不同组织间的表达模式。
Osmanthus serrulatus is an endemic Osmanthus fragrans species, which has a very high prospect of development and application. Using the Illumina HISeqTM2000 high-throughput sequencing platform, the authors performed deep sequencing and splicing assembly of the transcriptome of flower and bud of Flos Woodcell, constructing and predicting the gene expression profiles of different tissues of Fritillaria nivalis Its metabolic pathway. Transcriptome sequencing obtained a total of 16.94 G of valid data, and 92,798 unigenes were assembled de novo by Trinity, among which 35,851 unigenes were closely related to the Nr, Nt, Pfam, KOG, Swiss-Prot, KEGG and PFAM Public database comparison obtained gene function annotation information. From the KEGG metabolic pathway analysis, a total of 8,779 unigenes were involved in 262 metabolic pathways, of which 53 and 335 were involved in pigment and aroma metabolism, respectively. In addition, 6 genes involved in the biosynthesis of carotenoid, which were significantly differentially expressed in rhizhys and flower buds, were examined by real-time fluorescence quantitative PCR to verify their expression pattern in different tissues.