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运用聚合酶链反应 (PCR)技术 ,结合短串联重复序列 (STR)多态性标记的方法 ,对山东地区汉族 10 7例 2型糖尿病患者和 10 5例正常对照者 ,进行葡萄糖激酶 (GCK)基因及小肠脂肪酸结合蛋白 (FABP2 )基因的多态性分析。结果显示 GCK等位基因 A5与 2型糖尿病组呈正相关 (RR=15 .70 ,P<0 .0 0 1) ;GCK等位基因 A1、A3与 2型糖尿病组呈负相关 (RR=0 .2 8,P<0 .0 0 1;RR=0 .15 ,P<0 .0 0 1)。FABP2各等位基因频率在两组分布未见显著性差异。多因素非条件 L ogistic回归分析发现 ,2型糖尿病的发病与 GCK等位基因 A5呈显著正相关 ;与 GCK等位基因 A3呈显著负相关。年龄、甘油三酯、载脂蛋白 B及脂蛋白 a亦是 2型糖尿病发病的危险因素。认为 GCK基因多态性与山东地区汉族 2型糖尿病具有相关性 ,是 2型糖尿病的危险因素。FABP2 基因与 2型糖尿病未见相关性。
Using polymerase chain reaction (PCR), combined with short tandem repeat (STR) polymorphism markers, 107 cases of type 2 diabetes in Han nationality in Shandong and 105 normal controls were treated with glucokinase (GCK) Gene and small intestine fatty acid binding protein (FABP2) gene polymorphism analysis. The results showed that there was a positive correlation between GCK allele A5 and type 2 diabetes mellitus (RR = 15 .70, P <0.01). GCK allele A1, A3 was negatively correlated with type 2 diabetes mellitus (RR = 0. 2 8, P <0. 001; RR = 0 .15, P <0. 0 0 1). FABP2 allele frequencies in the two groups showed no significant difference. Multivariate non-conditional L ogistic regression analysis found that the incidence of type 2 diabetes was positively correlated with GCK allele A5 and negatively correlated with GCK allele A3. Age, triglycerides, apolipoprotein B and lipoprotein a are also risk factors for type 2 diabetes. It is considered that GCK gene polymorphism is associated with type 2 diabetes in Shandong Han people and is a risk factor for type 2 diabetes. FABP2 gene and type 2 diabetes no correlation.