目的采用反相高效液相色谱法测定天台乌药中乌药醚内酯。方法色谱柱为Symmetry C18柱(250 mm×4.6 mm,5.0μm);流动相为乙腈-水(57∶43);体积流量1 mL/min;检测波长235 nm。结果该方法下乌药醚内酯线性范围为0.051~2.55μg,平均回收率为99.96%。结论本法简便、经济,结果准确,可用于天台乌药的质量控制。 Objective To use RP-HPLC method to determine the Zanthoxylum-lactone lactone in Tiantaihua medicine. Methods The chromatographic column was a Symmetry C18 column (250 mm×4.6 mm, 5.0 μm); the mobile phase was acetonitrile-water (57:43); the volumetric flow rate was 1 mL/min; the detection wavelength was 235 nm. [Results] The linear range of nigerolide was 0.051~2.55μg and the average recovery was 99.96%. Conclusion This method is simple, economical and accurate. It can be used for the quality control of T. arborescens.