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分离脐血中的单个核细胞,以酸洗脱食管癌抗原肽负载获得分化成熟树突状细胞(DCs),检测其对特异性细胞毒T淋巴细胞(CTL)的诱导和体外杀伤效应的影响。结果发现,负载食管癌抗原肽的脐血DCs诱导的CTL对酸洗前食管癌细胞株T.Tn的杀伤率显著高于酸洗后组和各对照组(P均<0.05),而对无关肿瘤细胞株Hep2无显著杀伤作用。认为负载食管癌抗原肽的脐血DCs体外可诱导CTL产生特异性杀伤效应。
The mononuclear cells in cord blood were isolated and the differentiated mature dendritic cells (DCs) were obtained by acid-eluting esophageal cancer antigen peptides and their effects on the induction of specific cytotoxic T lymphocytes (CTLs) and the killing effect in vitro . The results showed that CTLs induced by cord blood DCs loaded with esophageal cancer antigen peptides were significantly higher than those of pickled and control groups (P <0.05), but not The tumor cell line Hep2 showed no significant killing effect. Cord blood DCs loaded with esophageal cancer antigen peptides are thought to induce CTLs to produce specific killing effects in vitro.