论文部分内容阅读
目的探讨噬血细胞综合征患儿外周血Th17、Th22及Treg细胞频数及相关细胞因子变化在HPS发生发展中的作用。方法收集本院自2014年收治的HPS初发患儿68例,其中缓解患儿55例,死亡13例,以同期健康体检儿童30例作为对照组,以流式细胞仪检测3组外周血Th17、Th22细胞及Treg细胞频数,以酶标仪检测患儿血浆中细胞因子IL-17、IL-21、IL-22及IL-35。结果 HPS初诊组外周血Th17、Th22细胞比例及其相关细胞因子IL-17、IL-21、IL-22较对照组、HPS缓解组均明显升高,差异均有统计学意义(P<0.05),而Treg细胞频数及其相关细胞因子IL-35较对照组、HPS缓解组均明显降低,差异有统计学意义(P<0.05)。结论 Th22、Th17细胞及其相关细胞因子IL-22、IL-17及IL-21在HPS患儿外周血中增高,而Treg细胞及其相关细胞因子IL-35比例下降,在HPS的发生发展中可能起着重要作用。
Objective To investigate the role of Th17, Th22 and Treg cell frequencies in peripheral blood and related cytokines in the development of HPS in children with hemophagocytic syndrome. Methods 68 cases of HPS new onset in our hospital were collected from 2014, including 55 cases of remission in children and 13 cases of death, and 30 cases of healthy children in the same period were taken as control group. Flow cytometry was used to detect Th17 , Th22 cells and Treg cells were detected by ELISA. The levels of cytokines IL-17, IL-21, IL-22 and IL-35 in children plasma were measured by ELISA. Results Compared with the control group and HPS remission group, the proportion of Th17 and Th22 cells in peripheral blood and the related cytokines IL-17, IL-21 and IL-22 in HPS newly diagnosed group were significantly increased (P <0.05) , While the frequency of Treg cells and related cytokines IL-35 were significantly lower than those in control group and HPS remission group (P <0.05). Conclusion Th22, Th17 cells and their related cytokines IL-22, IL-17 and IL-21 are increased in peripheral blood of HPS patients, while the proportion of Treg cells and related cytokines IL-35 is decreased. In the development of HPS May play an important role.