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目的:研究消痰散结方对体外肿瘤细胞诱导的血管内皮细胞生长的影响。方法:将不同浓度消痰散结方与胃癌 MKN-45细胞和体外培养的人脐静脉内皮细胞共同作用,用甲基噻唑基四唑(MTT)法测定人脐静脉内皮细胞数。结果:消痰散结方50μg/ml、100 μg/ml、200 μg/ml 能明显抑制胃癌 MKN-45细胞诱导的血管内皮细胞生长,与对照组比较差异有显著性(P<0.01);而消痰散结方对血管内皮细胞生长无显著影响。结论:消痰散结方能抑制胃癌 MKN-45细胞诱导的血管内皮细胞生长。
Objective: To investigate the effect of Xiaotan Sanjie Fang on the growth of vascular endothelial cells induced by tumor cells in vitro. METHODS: Different concentrations of Xiaotan Sanjie Fang were treated with gastric cancer MKN-45 cells and cultured human umbilical vein endothelial cells in vitro. The number of human umbilical vein endothelial cells was measured by methylthiazolyl tetrazole (MTT) method. RESULTS: The Xiaotansanjie 50μg/ml, 100 μg/ml, and 200 μg/ml could significantly inhibit the growth of vascular endothelial cells induced by gastric cancer MKN-45 cells, and there was significant difference compared with the control group (P<0.01). Xiaotan Sanjie Fang had no significant effect on the growth of vascular endothelial cells. Conclusion: Xiaotan Sanjie recipe can inhibit the growth of vascular endothelial cells induced by gastric cancer MKN-45 cells.