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目的了解陕西省流行性腮腺炎病毒株(mumps virus,MuV)的基因型别。方法采集腮腺炎病人咽拭子标本分离病毒,对分离到的MuV小疏水蛋白(small hydrophobic protein,SH)基因316个核苷酸片段进行逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)扩增,,对扩增产物进行序列测定和基因分型。结果从47份腮腺炎病人咽拭子标本中分离出腮腺炎病毒10株,分离率为21.3%,其中5株为F基因型,5株为G基因型。结论 F基因型、G基因型两种腮腺炎病毒在陕西省流行。
Objective To understand the genotype of mumps virus (MuV) in Shaanxi Province. Methods The virus was isolated from the pharyngeal swab specimens of mumps patients and the reverse transcription-polymerase chain reaction (PCR) was performed on the 316 bp of Mu hydrophobic small hydrophobic protein (SH) RT-PCR), sequence analysis and genotyping of amplification products. Results Mumps virus was isolated from throat swab samples of 47 mumps patients, with the isolation rate of 21.3%. Among them, 5 were F genotypes and 5 were G genotypes. Conclusions Both mumps virus of F genotype and G genotype are endemic in Shaanxi province.