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山东省1983年经卫生部考核,达到基本消灭丝虫病的标准。为了及时掌握我省基本消灭丝虫病后人群抗体的变化规律,为制定防治后期的措施提供依据,我们自1989年开始应用马来丝虫成虫冰冻切片抗原间接荧光抗体试验(IFAT)对原不同流行区基本消灭丝虫病后不同时间、不同人群进行了抗体监测。材料和方法一、抗原制备取雌性马来丝虫成虫,充分洗涤后,按冯祖梅等(1983)的方法制备。二、诊断血清羊抗人IgG荧光抗体诊断血清,为上海生物制品研究所和北京军事医学科学院生物制品研究所产品。三、监测对象 1.原高度流行区基本消灭丝虫病后8年的郯城
Shandong Province in 1983 by the Ministry of Health assessment, to basically eliminate filariasis standards. In order to keep abreast of the change rules of human antibodies after the basic elimination of filariasis in our province, and to provide the basis for the formulation of the measures for the prevention and control of the filariasis, we applied the indirect antibody test of frozen section antigen (IFAT) Antibiotics were monitored in different populations at different times after the epidemic area basically eradicated filariasis. Materials and methods First, the antigen preparation Take female adult Malayan worm, after full washing, according to Feng Zuomei et al (1983) Preparation. Second, the diagnosis of serum goat anti-human IgG fluorescent antibody diagnostic serum for the Shanghai Institute of Biological Products and Beijing Academy of Military Medical Biological Products Institute products. Third, the monitoring object 1. The original high degree of endemic areas to eliminate filariasis eight years after the Tancheng