应用免疫组化法对肾组织中淋巴细胞亚群进行测定,以探讨肾组织标本处理、固定液选用、单克隆抗体作用时间对染色结果的影响。并比较了四种免疫组化方法的优劣。结果认为冰冻切片的肾组织经丙酮固定,加高稀释度CD_4、CD_(?)、B 细胞单克隆抗体作用1.5小时,再以标记辣根过氧化物酶的兔抗鼠、猪抗兔免疫球蛋白相继作用后,再加兔PAP,即改良PAP 四层法最适用于肾组织中淋巴细胞的测定。 The immunohistochemical method was used to determine the lymphocyte subsets in the kidney tissue to explore the effects of treatment of renal tissue specimens, selection of fixative solution, and duration of monoclonal antibody on staining results. And compared the advantages and disadvantages of the four immunohistochemical methods. The results showed that frozen sections of kidney tissue were fixed with acetone, and were added with high dilution CD_4, CD_(?), B cell monoclonal antibodies for 1.5 hours, and then labeled with horseradish peroxidase-conjugated rabbit anti-rat, pig anti-rabbit immunity balls Following the sequential action of the protein, rabbit PAP is added, ie, the modified PAP four-layer method is most suitable for the determination of lymphocytes in renal tissue.