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目的评估大蒜多糖的益生元功能。方法以不同剂量的大蒜多糖灌胃健康雌性昆明小鼠,并以菊糖为阳性对照,采用微生物选择性培养分析和T-RFLP技术分析大蒜多糖对小鼠肠道微生态的影响,同时利用全自动血细胞分析仪来分析小鼠血脂代谢指标。结果连续灌胃21 d后,大蒜多糖和菊糖能够改善小鼠肠道微生态菌群,其中双歧杆菌数量显著增加(P<0.01),肠杆菌数量显著下降(P<0.05),而类杆菌的数量变化差异无统计学意义,T-RFLP结果也表现出同样的变化趋势。不同实验组小鼠血清中胆固醇及甘油三酯的含量和鼠胸腺和脾脏的重量与体重的比值差异无统计学意义,说明大蒜多糖对小鼠的免疫系统无副作用。结论本实验初步证实了大蒜多糖具有益生元功效。
Objective To assess the prebiotic function of garlic polysaccharides. Methods Different doses of garlic polysaccharide were instilled into healthy female Kunming mice and the inulin was used as a positive control. Microbial selective culture analysis and T-RFLP technique were used to analyze the effects of garlic polysaccharide on the gut microflora in mice. Automatic blood cell analyzer to analyze the indicators of lipid metabolism in mice. Results After 21 days of continuous gavage, garlic polysaccharide and inulin could improve the gut microbiota in mice, and the number of Bifidobacteria increased significantly (P <0.01) and the number of Enterobacteriaceae decreased significantly (P <0.05) There was no significant difference in the number of bacilli between the two groups. T-RFLP also showed the same trend. There was no significant difference in serum cholesterol and triglyceride content and the weight and body weight ratio between mouse thymus and spleen in different experimental groups, indicating that garlic polysaccharide has no side effects on the immune system of mice. Conclusion The experiment initially confirmed that garlic polysaccharide has a prebiotic effect.