目的探讨E1B55KDa缺陷腺病毒dl1520体外对鼻咽癌细胞的杀伤作用。方法dl1520感染不同鼻咽癌株后测定病毒增殖;观察细胞病变效应;MTT法测定细胞生长抑制作用。结果dl1520在鼻咽癌细胞中可复制增殖并有致细胞病变效应,MTT法测定感染复数为0.032、0.16、0.8、4、20、100时CNE2细胞生长抑制率分别为2.78%、7.41%、10.19%、24.07%、45.37%、67.59%。结论dl1520体外可在鼻咽癌细胞中复制并裂解杀伤肿瘤细胞。 Objective To investigate the killing effect of E1505Da-deficient adenovirus dl1520 on nasopharyngeal carcinoma cells in vitro. Methods The proliferation of different nasopharyngeal carcinoma strains was detected after infection with dl1520. The effects of cytopathic effect were observed. The cell growth inhibition was measured by MTT assay. Results dl1520 could replicate and induce cytopathic effect in nasopharyngeal carcinoma cells. The growth inhibition rates of CNE2 cells were 2.78%, 7.41% and 10.19%, respectively, when the number of infected cells was 0.032, 0.16, 0.8, 4, , 24.07%, 45.37%, 67.59%. Conclusion dl1520 can replicate and lyse tumor cells in vitro in nasopharyngeal carcinoma cells.