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目的：观察卡介菌多糖核酸（BCG - PSN）对 I 型变态反应模型小鼠腹腔巨噬细胞分泌 TNF -α的动态变化的影响。方法应用卵清蛋白注射雾化吸入致敏激发法建立 I 型变态反应小鼠模型。依据培养巨噬细胞的 RPMI -1640完全培养基中所含卡介菌多糖核酸浓度的不同进行分组，设非模型组（NG 组），模型给药组（0μl/ ml、20μl/ ml、40μl/ ml、80μl/ ml）共五组，作用时间分为12 h、24 h、36 h 三个时间点。实时荧光 PCR 法和酶联免疫吸附法测定巨噬细胞分泌 TNF -α的水平。结果实时荧光 PCR 法和酶联免疫吸附法测得 TNF -αmRNA 和 TNF -α模型组腹腔巨噬细胞较非模型组表达水平明显降低（ P <0.05）。而模型组随 BCG - PSN 浓度的升高 TNF -αmRNA 和 TNF -α表达量明显增高，差异有统计学意义（ P <0.05）。随作用时间延长，TNF -αmRNA 和 TNF -α表达量明显增高。结论 I 型变态反应小鼠腹腔巨噬细胞分泌 TNF -α水平降低，BCG - PSN 可增加巨噬细胞分泌 TNF -α的水平，参与拮抗 I 型变态反应，提示其在 I 型变态反应的临床治疗方面有重要作用。“,”Objective To observe the dynamic change of BCG - polysaccharide nucleic acid(BCG - PSN)on the production of TNF - αof peritoneal macrophages in type I allergy model mice. Methods Sensitization method was used to establish a mouse model of type I allergy by ovalbumin injection atomization inhalation. On the basis of cultured macrophages RPMI - 1640 complete culture of BCG - PSN concentration in containing different grouping,these models were divided into normal group(NG)and 0 μl/ ml,20 μl/ ml,40 μl/ ml,80 μl/ ml four different drugs concentration group. The role of time is 12h,24h and 36h three time points. The standard of TNF - α were measured by Quantitative Real- time PCR and ELISA. Results The TNF - α mRNA and TNF - α factor of peritoneal macrophages in model group are significantly lower than the normal group at expression level( P < 0. 05). The expression level of TNF - α mRNA and TNF - α factor in model group increased obviously with the rising of BCG - PSN concentration,differences are statistically significant( P < 0. 05),the expression level of TNF - α mRNA and TNF- α factor in model group increased obviously with the extension of time. Conclusion The level of TNF - α in type I allergy model mice perito-neal macrophages is lower. However,the BCG - PSN can increase that,which may play an important role in antagonizing I type allergic reaction, indicating that BCG - PSN has the significant potential in the clinical treatment of urticaria.