论文部分内容阅读
目的：观察比较人和动物肝脏感染肾综合征出血热（ＨＦＲＳ）病毒后病变的异同．方法：以原位分子杂交和免疫组化检测ＨＦＲＳ尸检及自然感染大鼠肝脏中病毒ＲＮＡ，病毒抗原及免疫球蛋白（Ｉｇ）的定位和分布及尸检肝脏中ｃ－ｍｙｃ基因．结果：尸检肝脏中病毒ＲＮＡ，病毒抗原和Ｉｇ的阳性率分别为８４．２１％（１６／１９），８８．２３％（１５／１７）和５３．３０％（８／１４），大鼠肝脏阳性率分别为９２．９０％（７９／８５），９３．１０％（２７／２９）和５４．５０％（１８／２９）；尸检肝脏ｃ－ｍｙｃ基因阳性率为５０．００％（８／１６）；尸检阳性者主要为死于休克期和重型病例；类似于人尸检肝脏中的病变未在大鼠肝脏中观察到．结论：与其它脏器相比，肝脏具有较高的病毒感染率．肝细胞异常Ｉｇ阳性可能是由病毒感染造成的肝细胞的可逆性损伤的体现，而肝细胞的坏死可能是损伤严重者．肝脏局部血液循环障碍是造成大片坏死的主要因素．Ｃ－ｍｙｃ基因的表达可能是肝脏抗损伤的一种反应．
Objective: To observe the similarities and differences of pathological changes of human and animal liver after hemorrhagic fever with renal syndrome (HFRS) virus infection. Methods: The in situ hybridization and immunohistochemistry were used to detect the localization and distribution of viral RNA, viral antigen and immunoglobulin (Ig) in the liver of post-mortem and self-infected HFRS autopsy specimens and autopsy c-myc gene in the liver. Results: The positive rates of viral RNA, viral antigens and Ig in the autopsy liver were 84.21% (16/19), 88.23% (15/17) and 53.30% (8/14), respectively The positive rates of c-myc gene in liver autopsy were 92.90% (79/85), 93.10% (27/29) and 54.50% (18/29), respectively. The positive rate of c- 16); positive autopsy mainly died of shock and heavy cases; lesions similar to human autopsy in the liver were not observed in rat livers. Conclusion: Compared with other organs, the liver has a high virus infection rate. Ig abnormal liver cells may be caused by the virus infection caused by reversible damage to the liver cells, and liver cell necrosis may be severe damage. Local liver blood circulation disorder is the main factor causing large necrosis. The expression of C-myc gene may be a response to liver injury.