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目的研究硼替佐米及阿糖胞苷序贯及联合用药对K562细胞的凋亡率的影响,同时观察硼替佐米是否经由PTPROt基因的表达上调促细胞凋亡。方法以慢性粒细胞白血病(CML)细胞的K562细胞为研究对象。不同用药组分别用药48 h,细胞选用噻唑蓝(MTT)法检测细胞增殖抑制率,采用流式细胞术观察细胞抑制率与凋亡率情况,同时观察PTPROt基因表达情况。结果联合应用硼替佐米及阿糖胞苷组与分别应用硼替佐米及阿糖胞苷组两两相比差异有统计学意义(P<0.05)。联合用药使细胞凋亡率增加,序贯先应用阿糖胞苷后硼替佐米的细胞凋亡率最高,与单用硼替佐米及阿糖胞苷组两两相比差异有统计学意义(P<0.05)。应用硼替佐米后PTPROt基因表达明显上调。结论联合硼替佐米及阿糖胞苷,对白血病细胞株K562细胞有协同增强肿瘤细胞凋亡作用,其作用机制可能与上调PTPROt基因表达相关。
Objective To study the effects of bortezomib and cytarabine sequential and combination on the apoptosis rate of K562 cells and to observe whether bortezomib up-regulates the apoptosis through the expression of PTPROt gene. Methods K562 cells of chronic myelogenous leukemia (CML) cells were studied. The different treatment groups were treated for 48 h, cells were treated with thiazolyl tetrazolium (MTT) assay inhibition rate of cell proliferation, cell inhibition rate and apoptosis rate were observed by flow cytometry, and PTPROt gene expression was observed. Results Bortezomib and cytarabine combined with bortezomib and cytarabine respectively showed significant difference (P <0.05). The combination of drugs increased the rate of apoptosis, sequential application of cytarabine after the highest rate of apoptosis of bortezomib, with bortezomib and cytarabine alone compared the two groups was statistically significant (P < P <0.05). PTPROt gene expression was significantly up-regulated after bortezomib administration. Conclusions Combination of bortezomib and cytarabine can synergistically enhance the apoptosis of leukemia cell line K562 cells. The mechanism may be related to up-regulation of PTPROt gene expression.