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目的:建立HPLC法同时测定中药退黄外洗液中栀子苷、柚皮苷含量。方法:色谱柱:Agilent SB-C_(18)柱(250 mm×4.6mm,5μm),流动相:以乙腈-水梯度洗脱,流速1.0 ml·min~(-1),检测波长,238 nm和283 nm。结果:栀子苷在107～1 075 ng范围内线性关系良好r=0.999 9,平均回收率为99.3%,RSD=0.30%(n=6);柚皮苷在119～1 190 ng范围内线性关系良好r=0.999 9,平均回收率为99.2%,RSD=0.24%(n=6)。结论:操作简便,准确可靠,可作为中药退黄外洗液的质量控制。
Objective: To establish a HPLC method for the simultaneous determination of geniposide and naringin in the Chinese medicine dandruff lotion. Methods: The column was Agilent SB-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was eluted with acetonitrile-water gradient at a flow rate of 1.0 ml · min -1. The detection wavelength was 238 nm And 283 nm. Results: The calibration curve of geniposide was linear in the range of 107 ~ 1 075 ng (r = 0.999 9) with an average recovery of 99.3% and a RSD of 0.30% (n = 6). Naringin was linear over the range of 119-1 190 ng Good correlation was r = 0.999 9 with an average recovery of 99.2% and RSD = 0.24% (n = 6). Conclusion: The operation is simple, accurate and reliable, which can be used as the quality control of the Chinese medicine digesting external washings.