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目的分析年龄、性别对鸡蛋过敏人群中主要过敏原反应性的影响,初步探索我国鸡蛋过敏儿童卵类粘蛋白的主要抗原表位。方法以卵类粘蛋白、卵白蛋白、卵转铁蛋白纯品为抗原,采用免疫斑点印迹,检测21例鸡蛋过敏儿童血清特异性免疫球蛋白E(SIgE)与蛋白反应性,分析年龄、性别因素对过敏原反应性的影响;应用DNAStar生物分析软件,预测并合成卵类粘蛋白抗原表位肽段,采用免疫斑点印迹检测抗原表位与鸡蛋过敏儿童血清的反应性。结果 A(≤1岁)组、B组(2~3岁包括3岁)、C组(>3岁)的卵类粘蛋白反应率差异有统计学意义(P<0.05),卵白蛋白、卵转铁蛋白反应率各组间均差异无统计学意义(P>0.05);性别对各过敏原的反应率差异无统计学意义(P>0.05);卵类粘蛋白抗原表位反应率较低。结论卵类粘蛋白sIgE在不同年龄段存在差别,卵白蛋白sIgE在21例患者均可检测出,卵转铁蛋白s Ig E在各年龄段无明显差别;应用DNAStar生物软件预测的卵类粘蛋白抗原表位,在天津地区反应性低,不是该地区主要抗原表位。
Objective To analyze the effect of age and sex on the major allergenicity in egg-allergic subjects and to explore the main epitopes of egg-like mucins in children with egg allergy in China. Methods Serum specific immunoglobulin E (SIgE) and protein reactivity in 21 cases of egg-allergic children were detected by immunofluorescent dot blotting using egg-like mucin, egg albumin and egg transferrin as the antigen. Age, gender On the allergen reactivity; using DNAStar bioanalytical software to predict and synthesize the peptide epitopes of ovomucoid epitopes, using immunoblot blot detection antigen epitopes and egg-allergic children serum reactivity. Results The response rates of ovum mucin in group A (≤1 year old), group B (aged 3 to 3 years old) and group C (> 3 years old) were significantly different (P <0.05) There was no significant difference in the response rate of transferrin between the groups (P> 0.05). There was no significant difference in the response rate of allergens between sexes (P> 0.05). The response rate of egg mucin epitopes was low . Conclusion Ovary mucin sIgE is different in different age groups. Ovalbumin sIgE can be detected in all 21 cases. Ovotransferrin s Ig E has no significant difference in all age groups. Ovary mucin predicted by DNAStar software Epitopes, which are less reactive in Tianjin, are not the major epitopes in the region.