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目的:探讨靛玉红甲肟(IRO)对人喉癌Hep-2细胞增殖的影响及机制。方法:MTT法检测不同浓度、不同作用时间IRO对Hep-2细胞增殖活性的影响。流式细胞仪检测IRO对Hep-2细胞周期的影响。Western法检测IRO对Hep-2细胞CDK4和cyclinD1蛋白表达的影响。结果:MTT结果发现IRO对Hep-2细胞具有明显的增殖抑制作用,且表现为剂量依赖性(P<0.01)。流式检测发现,以10μmol/L的IRO处理Hep-2细胞后,细胞阻滞于G0/G1期,不同浓度组间差异存在显著性(P<0.01)。Western结果发现IRO对Hep-2细胞CDK4和cyc-linD蛋白的表达有抑制作用(P<0.01)。结论:IRO具有抑制人喉癌Hep-2细胞增殖的作用,其机制与阻滞细胞于G0/G1期有关。
Objective: To investigate the effect and mechanism of indomethacin (IRO) on the proliferation of human laryngeal carcinoma Hep-2 cells. Methods: MTT assay was used to detect the proliferation of Hep-2 cells with IRO at different concentrations and different time. Flow cytometry was used to detect the effect of IRO on Hep-2 cell cycle. Western blot was used to detect the effect of IRO on the expression of CDK4 and cyclinD1 in Hep-2 cells. Results: MTT results showed that IRO had a significant inhibitory effect on Hep-2 cells in a dose-dependent manner (P <0.01). Flow cytometry showed that cells treated with IRO at 10μmol / L blocked G0 / G1 phase, and there was significant difference between different concentrations of IRO (P <0.01). Western results showed that IRO inhibited the expression of CDK4 and cyclinD protein in Hep-2 cells (P <0.01). CONCLUSION: IRO can inhibit the proliferation of human laryngeal carcinoma Hep-2 cells. The mechanism is related to the arrest of cells in G0 / G1 phase.