论文部分内容阅读
目的:建立大鼠中性粒细胞(PMN)与滑膜细胞(RSC)的粘附模型。方法:MTT比色法。结果:500U·mL-1rhTNFα与IL1刺激RSC12h对PMN有明显的粘附作用,MTTPMN细胞数与540nm吸光度值有良好的线形相关性;氢化考的松(hydrocortisone,Hy)和美洛昔康(meloxicam,Melo)均可抑制PMN与500U·mL-1rhTNFα/或IL1诱导的RSC粘附,提示Hy和Melo治疗类风湿性关节炎(RA)可能与其抑制PMN与RSC粘附作用有关。结论:以MTT比色法为基础建立的检测大鼠PMN与RSC的粘附模型,可用于PMN与RSC粘附抑制剂的筛选。
Objective: To establish an adhesion model of rat neutrophil (PMN) and synoviocyte (RSC). Methods: MTT colorimetric method. Results: The adhesion of PMN to RSC12h was stimulated by 500 U · mL-1 rhTNFα and IL1, and there was a good linear correlation between MTTPMN cell number and absorbance at 540 nm. Hydrocortisone (Hy) and meloxicam Melo) could inhibit the adhesion of PMN to 500U · mL-1 rhTNFα / IL1-induced RSC, suggesting that Hy and Melo treatment of rheumatoid arthritis (RA) may be related to its inhibition of adhesion of PMN and RSC. Conclusion: The model of adhesion between PMN and RSC established by MTT colorimetric assay can be used to screen PMN and RSC adhesion inhibitors.