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以表型差异明显的两个青花菜(Brassica oleracea var.italica)高代自交系为材料杂交获得F1,通过游离小孢子培养技术和人工自交方法,分别构建了由176个DH系组成的DH群体和由176个单株组成的F2群体。采用性状分离分析和群体遗传多样性分析方法,对两个群体13个主要农艺性状的平均值、变异系数及性状分离区间等遗传表现进行分析。结果表明:13个主要农艺性状均是受多基因控制的数量性状,在DH群体和F2群体中均发现了正向和负向两个方向的超亲基因型,两群体的平均变异系数和遗传多样性指数相近。可见,通过DH技术育种不仅可以加快亲本的纯化进程,而且可获得广泛分离的育种资源,从而提高育种效率。
F1 hybrids were obtained from two inbred lines of high-yielding Brassica oleracea var.italica with obvious phenotypic differences. F1 was constructed by means of free microspore culture and artificial self-fertilization. DH population and an F2 population of 176 individuals. The genetic analysis of 13 major agronomic traits, the coefficient of variation and the segregation interval of traits of 13 populations were conducted by trait segregation analysis and population genetic diversity analysis. The results showed that all the 13 major agronomic traits were quantitative traits controlled by multiple genes. Both the positive and negative directions were found in both DH and F2 populations. The average coefficient of variation Diversity index is similar. It can be seen that DH breeding can not only speed up the process of purification of parents, but also obtain widely separated breeding resources to improve breeding efficiency.