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为了创制具有不同黄花叶病抗性的大麦突变体材料,对大麦品种花30的干种子和游离小孢子分别采用物理和化学诱变处理,结合小孢子培养技术获得了一批纯合稳定的加倍单倍体(double haploid,DH)株系,并利用黄花叶病种植鉴定圃进行突变体DH株系的大田抗性筛选与鉴定,比较2种诱变方式结合小孢子培养的诱变效果。结果表明:以400 Gy剂量60Co-γ射线处理干种子诱变方式对小孢子培养的愈伤组织诱导能力和绿苗成活率有较大影响,获得的大部分株系(84.2%)对黄花叶病表现感病(感病或高感),未筛选到比亲本抗性增强(高抗)的材料;以3 mg·L-1剂量EMS处理游离小孢子诱变方式对小孢子培养相对影响较小,筛选到少量(5.5%)比亲本抗性增强(高抗)的株系,只有近1/3的材料(31.1%)表现为感病(感病或高感),进一步鉴定筛选到2份高感和1份高抗黄花叶病的大麦突变体材料。综上所述,EMS处理游离小孢子结合小孢子培养诱变效果较好。本研究为利用大麦黄花叶病抗性突变体材料进行其抗病机理研究奠定了基础。
In order to create barley mutant materials with different resistance to yellow mosaic disease, physical and chemical mutagenesis of dry seeds and free microspores of flower 30 of barley varieties were carried out respectively. Combined with microspore culture techniques, a batch of homozygous stable doubled The haploid (double haploid, DH) lines were selected and screened for the field resistance of mutant DH lines by using yellow mosaic disease identification. The mutagenic effects of two mutagenesis methods combined with microspore culture were compared. The results showed that the treatment of 60 Gy γ-rays with 400 Gy dose of dry seeds had a significant effect on the induction ability of callus and the survival rate of green shoots. Most of the obtained lines (84.2% The disease was susceptible (susceptible or susceptible), and no material with stronger resistance (higher resistance) than that of the parent was screened. The relative impact of microspore culture on the treatment of free microspore by 3 mg · L-1 dose of EMS Only a small amount (5.5%) of the selected lines had stronger resistance (high resistance) than that of the parents, and only one third of the materials (31.1%) showed susceptible (susceptible or susceptible) lines, and 2 A sense of proportion and a high resistance to yellow mosaic disease barley mutant material. In summary, EMS treatment of microspores combined with microspore culture mutagenesis better. This study lays the foundation for the research of the disease-resistant mechanism of barley yellow mosaic disease-resistant mutants.