论文部分内容阅读
目的研究蜂胶黄酮(pinobanksin-3-acetate,PB3A)对体外培养的大肠癌SW480细胞中G蛋白信号转导调节因子2(regulator of G-protein signaling 2,RGS2)和GTP结合丝裂原诱导蛋白(GTP binding protein overexpressed in skeletal muscle,GEM)基因转录和蛋白质表达水平的影响,探讨PB3A的抗肿瘤作用机制及其相关信号通路。方法通过实时荧光定量RT-PCR和蛋白印迹法检测100 mg/L PB3A作用下人大肠癌细胞SW480中RGS2和GEM基因的转录和表达情况。结果 100 mg/L PB3A药物的干预诱导SW480细胞出现悬浮的细胞碎片、细胞体缩小、变圆、皱缩。药物干预之后,SW480细胞中RGS2和GEM基因在mRNA和蛋白水平上调表达。实验组与对照组比较,RGS2和GEM基因的mRNA转录水平差异有统计学意义(P<0.01),蛋白质表达水平差异有统计学意义(P<0.05)。根据RT-PCR检测结果,利用Spearman相关性分析检测出RGS2和GEM高度正相关(r=0.929,P<0.01)。结论 PB3A干预大肠癌SW480细胞后使RGS2和GEM基因在转录和翻译水平上上调表达。PB3A的抗大肠癌作用机制可能与RGS2和GEM基因的上调表达有关。
OBJECTIVE: To investigate the effect of pinobanksin-3-acetate (PB3A) on the expression of G-protein signaling 2 (RGS2) and GTP-associated mitogen-inducible protein GTP binding protein overexpressed in skeletal muscle, GEM) gene transcription and protein expression levels, to explore the anti-tumor mechanism of PB3A and its related signaling pathways. Methods The transcription and expression of RGS2 and GEM gene in SW480 cells were detected by real-time fluorescent quantitative RT-PCR and Western blotting. Results Suspension of cell debris was observed in SW480 cells induced by 100 mg / L PB3A, and the cell bodies were reduced, rounded and wrinkled. After drug intervention, RGS2 and GEM genes were upregulated at mRNA and protein levels in SW480 cells. Compared with the control group, the mRNA levels of RGS2 and GEM in the experimental group were significantly different (P <0.01), and the protein expression levels were significantly different (P <0.05). According to the results of RT-PCR, Spearman correlation analysis showed that RGS2 and GEM were highly correlated (r = 0.929, P <0.01). Conclusion PB3A can up-regulate the expression of RGS2 and GEM gene at transcription and translation levels in SW480 cells. The anti-tumor mechanism of PB3A may be related to the up-regulation of RGS2 and GEM genes.