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大蒜在生物体内有多种作用,如降低血清和肝中胆固醇含量,抑制血小板凝集以及抗菌活性。本文通过检验硫代巴比妥酸反应物(TBA-RS)和荧光物质形成的变化以及微粒体过氧化过程中膜的流动性改变来测定大蒜提取物在微粒体脂质过氧化中的作用。肝微粒体的制备采用Itoh方法(ArchBiochem Biophs 1988,264:184～191)用体重170～250g雄性Wister大鼠制得。大蒜提取物是将400ml 20％乙醇加至200g捣碎的大蒜鳞茎中,在4℃下真空减压浓缩,然后冷冻干燥,得30mg大蒜提取物。
Garlic has a variety of effects in the body, such as reducing serum and liver cholesterol levels, inhibiting platelet aggregation and antibacterial activity. In this paper, the role of garlic extracts in microsomal lipid peroxidation was determined by examining the changes in the formation of thiobarbituric acid reactants (TBA-RS) and fluorescent substances and the membrane fluidity changes during microsomal peroxidation. Preparation of liver microsomes was performed using the Itoh method (Arch Biochem Biophs 1988, 264: 184-191) with male Wister rats weighing 170-250 g. The garlic extract was prepared by adding 400 ml of 20% ethanol to 200 g of chopped garlic bulbs, vacuum concentration at 4° C., and freeze-drying to obtain 30 mg of garlic extract.