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目的探讨胃癌中乙酰肝素酶(heparanase)的表达及DNA含量与胃癌临床病理特征的关系。方法应用流式细胞术检测50例胃癌组织、25例癌旁组织和10例正常组织中乙酰肝素酶的表达和DNA含量。结果heparanase在胃癌组、癌旁组、正常组中表达量(FI值)分别为1.86±0.44,1.22±0.45,1.00±0.21,3组间差别有显著性(P<0.05),heparanase在胃癌中的阳性表达率为96%,明显高于癌旁和正常胃黏膜(P<0.01),且与胃癌的浸润深度(P<0.05)、TNM分期(P<0.01)和淋巴结转移相关(P<0.01)。3组的DI值分别为1.26±0.14,1.05±0.14,1.00±0.11,3组间差别有显著性(P<0.05),且与肿瘤的分化程度和淋巴结转移相关(P<0.01)。Heparanase表达的FI值与DI值呈正相关关系(r=0.536,P<0.01)。结论Heparanase在肿瘤的浸润和转移中扮演重要的角色,DNA含量与肿瘤的恶性程度密切相关。heparanase和DNA含量可能是评价胃癌恶性程度和预后预测的重要指标。
Objective To investigate the expression of heparanase and the relationship between DNA content and the clinicopathological features of gastric cancer. Methods The expression and DNA content of heparanase in 50 cases of gastric cancer, 25 cases of paracancerous tissues and 10 cases of normal tissues were detected by flow cytometry. Results The expression levels of heparanase in gastric cancer group, paracancer group and normal group were 1.86 ± 0.44, 1.22 ± 0.45 and 1.00 ± 0.21, respectively (P <0.05). Heparanase in gastric cancer (P <0.01). The positive rate was 96%, which was significantly higher than that in paracancer and normal gastric mucosa (P <0.01) ). The DI values in the three groups were 1.26 ± 0.14, 1.05 ± 0.14 and 1.00 ± 0.11, respectively. The differences among the three groups were significant (P <0.05), and were correlated with tumor differentiation and lymph node metastasis (P <0.01). The FI value of Heparanase was positively correlated with DI (r = 0.536, P <0.01). Conclusion Heparanase plays an important role in tumor invasion and metastasis. The DNA content is closely related to the malignancy of the tumor. Heparanase and DNA content may be important indicators to evaluate the degree of malignancy and prognosis of gastric cancer.