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目的建立高效液相色谱法测定消增颗粒中淫羊藿苷的含量方法。方法采用HP1100高效液相色谱仪(包括G1314A可变波长紫外检测器,HP化学工作站,G1322A真空脱气机,HP1100四元泵),Hypersil ODS柱(4.6mm×250mm,5μm)。流动相:乙腈-水(30∶70),流速:1.0mL.m in-1,检测波长:270 nm,进样量:20μl。结果淫羊藿苷在0.16~4.0μg范围内呈现良好的线性关系,回收率为98.6%(n=5),日内平均RSD为1.7%,日间平均RSD为2.1%。结论该法简便、准确、具有专属性,可用于测定消增颗粒淫羊藿苷的含量。
Objective To establish a HPLC method for the determination of icariin in Xiaogao particles. Method HP1100 high-performance liquid chromatograph (including G1314A variable wavelength UV detector, HP ChemStation, G1322A vacuum degasser, HP1100 quaternary pump), Hypersil ODS column (4.6mm x 250mm, 5μm). Mobile phase: acetonitrile-water (30:70), flow rate: 1.0 mL.m in-1, detection wavelength: 270 nm, injection volume: 20 μl. As a result, icariin showed a good linear relationship in the range of 0.16 to 4.0 μg, with a recovery rate of 98.6% (n=5), an intraday mean RSD of 1.7%, and an intraday RSD of 2.1%. Conclusion The method is simple, accurate and specific. It can be used to determine the content of icariin in Xiaogao particles.