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目的:建立人腮腺多形性腺瘤有限细胞系。方法:取腮腺多形性腺瘤患者原发肿瘤进行原代培养。观察细胞的形态特征,绘制细胞生长曲线,采用免疫细胞化学检测细胞内特异性抗原标志物,对细胞进行鉴定。结果:多形性腺瘤细胞体外培养时间约为80d,传代至16代,细胞呈短梭形、多边形,肿瘤性腺上皮细胞角蛋白弱阳性,肿瘤性肌上皮细胞肌动蛋白阳性、波形蛋白阳性。结论:多形性腺瘤细胞在体外培养连续传代,为永生化腮腺多形性腺瘤细胞系的建立奠定了基础。
Objective: To establish a limited human parotid gland adenoma cell line. Methods: Primary tumors of patients with parotid pleomorphic adenoma were primary cultured. Morphological characteristics of the cells were observed, and cell growth curves were drawn. Immunocytochemistry was used to detect intracellular specific antigenic markers and the cells were identified. Results: The culture time of pleomorphic adenoma cells in vitro was about 80 days. The cells were passaged to 16 passages. The cells were short fusiform, polygonal, weakly positive for keratin glandular epithelial cells and positive for actin in tumorous myoepithelial cells. Conclusion: The continuous passage of pleomorphic adenoma cells in vitro can lay a foundation for the establishment of immortalized parotid gland adenoma cell line.