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目的用复制大鼠脑缺血再灌注动物模型，研究细胞内外钙平衡紊乱与氧自由基在脑缺血再灌注损伤中的作用。方法测定假手术组、缺血３０ｍｉｎ再灌注６０ｍｉｎ生理盐水（ＮＳ）处理组和单唾液酸四己糖神经节苷脂（ＧＭ１）处理组海马脑组织线粒体钙（ｍｉｔｏｃｈｏｈｄｒｉａｃａｌｃｉｕｍ，ＭＣａ）、钙调素（ｃａｌｍｏｄｕｌｉｎ，ＣａＭ）、丙二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）含量变化。结果缺血３０ｍｉｎ再灌注６０ｍｉｎＮＳ处理组ＭＣａ、ＣａＭ、ＭＤＡ含量显著升高（Ｐ＜００１），用ＧＭ１处理后则上述指标明显改善。结论急性脑缺血再灌注后有细胞内外钙平衡紊乱和氧自由基代谢障碍发生，且相互协同作用
OBJECTIVE: To investigate the effects of intracellular free calcium balance and oxygen free radicals on cerebral ischemia-reperfusion injury in a rat model of cerebral ischemia-reperfusion. Methods The changes of mitochondrial calcium (MCa) and calmodulin (MMP) levels in the hippocampus of the sham operation group, the NS group and the GM1 group at 60 min after ischemia for 30 min were measured. calmodulin, CaM) and malondialdehyde (MDA). Results The levels of MCa, CaM and MDA were significantly increased after 30 min ischemia / reperfusion (P <001). The GM1 treatment significantly improved the above parameters. Conclusions After acute cerebral ischemia-reperfusion, intracellular and extracellular calcium balance disorders and oxyradical metabolism disorders occur and they act synergistically with each other