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目的:实验性变态反应性神经炎(EAN)是一类T细胞介导的周围神经系统的自身免疫病,可用牛坐骨神经加完全氟氏佐剂诱导而成。本文研究趋化因子mRNA在实验性变态反应性神经炎(EAN)中的表达并探索其可能的作用。方法:用兔坐骨神经匀浆免疫Wistar大鼠,诱导格林巴利综合症(GBS)的动物模型EAN;采用地高辛标记的寡核苷酸探针检测EAN病变神经组织浸润细胞上趋化因子单核细胞趋化蛋白-1(MCP-1)及巨噬细胞炎性蛋白-1β(MIP-1β)mRNA表达情况。结果:MCP-1mRNA在临床症状出现前1-2天(14天)水平最高,随后逐渐下降;MIP-1 βmRA在临床症状出现前1-2天水平开始升高,在临床症状达到高峰时(21天)最高,进入恢复期后降至基础水平。结论:趋化因子在EAN的炎性细胞迁移及浸润进入神经细胞过程中起到重要作用。
OBJECTIVE: Experimental allergic neuritis (EAN) is a type of T cell-mediated autoimmune disease of the peripheral nervous system that can be induced by bovine sciatic nerve plus complete Freunds adjuvant. This article studies the expression of chemokine mRNA in experimental allergic neuritis (EAN) and explores its possible role. Methods: Wistar rats were immunized with rabbit sciatic nerve homogenate to induce EAN in animal model of Guillain-Barre syndrome (GBS). Digoxigenin-labeled oligonucleotide probe was used to detect the expression of chemokines The expression of MCP-1 and MIP-1β mRNA was detected by RT-PCR. Results: The levels of MCP-1 mRNA in the first 1-2 days (14 days) before clinical symptoms appeared and then decreased gradually. MIP-1 βmRA began to rise 1-2 days before the onset of clinical symptoms. When the clinical symptoms peaked ( 21 days) the highest, after entering the recovery period dropped to the basic level. CONCLUSION: Chemokines play an important role in inflammatory cell migration and infiltration of EAN into neural cells.